Measure more accurately. 03 pM 384-well: 0. Read More. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. Can read any standard cuvette, micro cuvette, test tube and 96-well or UV-transparent microplate in the wavelength range of 190-1000 nm. g. Cells were washed three times with calcium wash buffer using an AquaMax 4000 Microplate Washer with 96- or 384-well cell wash head. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 µL per well. Published Dec 16, 2020 | Updated Dec 16, 2020. It includes both shaking and incubation functions, and is compatible with 96- well microplates. Reconstitution of. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. By far the most compact reader on the market, the footprint of the reader is almost as small as the plate itself. Working in microplates allows for smaller assays that use less sample and reagent and increases throughput. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Making it possible to perform kinetic analysis of solid. With various on-board features and capabilities,. Other features and benefits include: Eliminate changing out filters. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. Type: Microplate Reader. Shaking: Linear and Orbital. Align the two slots on the bottle holder with the two pegs on the rail. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. The concentration of total free thiols in the protein. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one systemComputer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit (signal 3X SD of baseline) 8. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. 03 pM. The VersaMax Absorbance Microplate Reader measures the optical density (OD) of samples at selected wavelengths in a number of reading modes. Molecular Devices SpectraMax 384 Plus Microplate Reader. The Molecular Devices Versamax Microplate Reader INCLUDED:. All stand-alone instrument functions can be controlled using the software. • 0. #W50-100) SpectraMax® ABS Plus Microplate Reader. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and time-resolved fluorescence (50-1450 µsec, 200 µsec increments) measurements in 6, 12, 24, 48, 96, and 384 well microplates or in. Up to 70% savings in overall assay time can be gained when using the Cell Cytotoxicity assay (Figure 1). Devices® SPECTRAmax 190 microplate reader at a wavelength of 600 nm. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. 1 pM 1536-well: 0. Materials. Please refer to the Obsolete Microplate Reader Models for more information. The FilterMax F5 can read 6, 12, 24, 48, 96, 384, 1536-well. 96-well-plate-scan-time-2-colors-1-site-well. g. 5 mm. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. Microplate luminescent reader assay for ERK activity. Published Dec 16, 2020 | Updated Dec 16, 2020. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Temperature Control: ambient from +4 to 45C. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The device was able to achieve 3σ limits of detection of ca. Related Products: Low Binding Plates. Compare this item. The SpectraDrop Microplate’s slide design eliminates the need for calibration and gives consistent well-to-well reads with CVs below 5%. 0 fmol/well FITC 200 µL in 96 wells. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec. Small but mighty absorbance plate reader •ffordable monochromator-based plate reader with linearity A of up to 3 OD • orks with 96- and 384-well plates as well as cuvettes W (ABS Plus) • Option to run samples as low as 2 μL with the SpectraDrop™ Micro-Volume Microplate • Runs various assays within the Vis/UV-Vis range such asI tried Nunc Edge 2. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. 19 H mm that meet microplate industry standards. we demonstrate how Molecular Devices microplate readers with luminescence detection mode provide superior sensitivity and ease of use for reliable mycoplasma detection using MycoAlert. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. The absorbance at 412 nm was measured by a SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA) at 30 °C. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. Instrument settings can be saved as a protocol file and used repeatedly for reading differentThe Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of Multi-mode microplate readers with fluorescence detection. #N207) LAL Reagent Water (Lonza cat. The SpectraMax ABS. (Molecular Devices cat. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C. 24, 48, 96, 384 Well Plates; Wavelength(s): 250 - 850 nm; Specifications/Features: Shaking, Temperature control, Cuvette port; Read More. in space. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. microplate readers. book Page 9 Wednesday, July 7, 2010 2:09 PM. 63. Pipet 100-200 µL of deionized water into each well of a clean 96-well microplate. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96- or 384-well head. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. A single-mode plate reader is a good starting point. SpectraMax Plus 384Microplate is validated by molecular devices for the FLIPR reader. Limit of Detection: < 20 attomol ATP per well Dynamic range > 9 orders of magnitude. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. At 5 seconds to read a 96-well endpoint assay, the EMax Microplate Reader has both the speed and performance to carry a heavy workload. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs). A single-mode plate reader is a good starting point. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. style. Bulk Kit (10-plate size): Molecular Devices P/N R8341; HeLa cells (ATCC P/N CCL-2) Staurosporine (Sigma P/N S5921) 96-well black, clear-bottom microplates (Corning P/N 3904) SpectraMax fluorescence microplate reader; Methods. Lucid Scientific. 3% in white 96- and 384-well microplates Time-Resolved Fluorescence Performance (M4, M5, M5e only) Reading capabilities Top or bottom of a microplatethe iD5 reader’s filter system. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data analysis with SoftMax® Pro Software Optimal performance with validation tools Small but Mighty The IC design and microplate construct represents a major advancement to state-of-the-art CMOS devices for biological measurement, where the large majority are formed in single well constructs 12. Standard SBS 96 and 384 well plates (128. With optimized reagents, option to operate the. Biochemical characterization was conducted, using GEN III MicroPlate™ Biolog identification system and confirmed with molecular identification via 16S rDNA gene. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. 2. When reading at wavelengths. About;. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. FlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Click “Next”, and the reader will start scanning the four corner wells; a progress indicator will appear: fter the reader finishes scanning the four corner wells, you will see a screen with an image of the upper left corner A well and a pink crosshair. 05. 96-well solid bottom, clear polystyrene microplates (Greiner cat. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. SmartReader 96: Designed for optimal ELISA Testing. Microplate luminescent reader assay for ERK activity. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. Look for a washer which can handle a variety of plate types, including deep well plates and 96-, 384- and 1536-well plates. After 24 hours, cells were trypsinized and seeded at 100 μL/well into a 96-well plate starting at 30,000 cells/well with subsequent 2-fold dilutions. The Perkin Elmer Wallac 1420. 54: 1. ZERO BIAS - scores, article reviews, protocol conditions and moreThe reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. Materials. Molecular Devices 1311 Orleans Drive Sunnyvale, California 94089 Telephone: +1-408. iD5. Four wash/rinse bottles are included and configurable for both 96- and 384. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Elisa Plate Readers and Microplate Readers at Biocompare. Omnia® Compatible Microplate Reader Documentation Version No. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Page: 1/4. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Detergent Compatible Bradford assay. Compare plate readers SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. 96-well microplate at 200 µL/well. Dynamic range > 9 orders of magnitude. measurements in compatible Molecular Devices® microplate readers. Measure assay absorbance values quickly with minimal background noise on the SpectraMax iD5 reader. Fluorescence Microplate Reader Comparison Chart. Reactions catalyzed by firefly and *Renilla luciferases. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. tecHnical SPecificationS Performance Specifications Measurement range: 400–750 nm 0–4. Or scan up to 384 wells at any time to determine the best assay conditions. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. 2. emax and Vmax microplate readers. 1536-well: 0. ZERO BIAS. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise. both cuvette-based and microplate reader applications SpectraMax Mini Multi-Mode Microplate Reader. The EMax Plus Microplate Reader measures 96-well plates and comes with eight standard filter modes to cover the entire visible range, for applications such as protein quantification, cell viability, cell proliferation and ELISA. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. Optical System: High energy xenon flash lamp. +49-89/96-05-88-0 fax +49-89/9-62-02-34-5 Japan molecular devices Japan, osaka. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. $489. Molecular Devices 推出了FlexStation® 3 多功能读板机系统。FlexStation 3 把 SpectraMax M5e 和 8 道 / 16 道的移液 系统整合在一起成为体积小巧功能强大的 读板机系统。该整合系统不但为用户提供 了多功能检测平台，还增加了液体处理的 通量和提升了检测生化和细胞动. M3-M5e_manual. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. The UV-Vis SpectraMax 190 Microplate Reader for Quantification of DNA and endpoint, kinetic, and spectral scanning analyses. Triplicates of each BSA concentration were made for this assay plate. " A versatile UV/VIS spectrophotometer for both 96- and 384-well microplates as well as cuvette. Other technologies such as imaging. Condition: Used - Good. 2 OD at 200 nm; this decreased to 0. SpectraMax Microplate Readers. In May 2011, Molecular Devices SpectraMax M5e reader, chosen for its durability, became the first microplate reader . Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs) Specifications/Features: Shaking, Temperature control, Injector. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. 96, 384, 24, 12, 6 well types. The procedure enables direct translation of any published equation. Retrieving. Multi-Mode Microplate Reader for . reagent basin for ease of pipetting into. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. The SpectraMax® iD5 Multi-mode Microplate Readers measure absorbance, fluorescence, and luminescence. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aSpectraMax Microplate Readers. (FP) configured microplate readers. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 1. It also allows for the elimination of standard curves. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. 0 OD at. The plate reader optics are tunable for. for 96- and 384-well microplates • Detect dim samples with unsurpassed sensitivity • Avoid saturation issues with nine orders of dynamic range • Enable robust performance with autorinse injectors • Validation and compliance tools SpectraMax® L Microplate . Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. robotics-automation-compatible. 6. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader s with all leading partner robots. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. 020 fmol 0. , Sigma cat. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn moreThe Molecular Devices microplate reader portfolio comprises various instruments, which differ regarding the design of the heating elements, as well as temperature control, and are therefore divided into three categories as shown in Table 1. changes in OD 600 were measured by periodically transferring 100-µL aliquots of each bacterial culture to a single well of a 96-well microplate and measuring absorbance at 600 nm using the SpectraMax iD3 reader and SoftMax Pro Software. Its software interface is easy to learn and use, and lets you control the instrument from almost any device –. The IC design and microplate construct represents a major advancement to state-of-the-art CMOS devices for biological measurement, where the large majority are. This reliable model allows for accurate wavelength detection in visible spectrum without the need for filters. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. A 1:10 serial dilution of ATP was prepared in medium. 2600A October 2023. # 0310-5218T). Quantitate proteins rapidly with a 5-minute, room-temperature incubation. (a) HCT116 cells expressing hyBRET-ERK were plated in 96-well plates and treated with serially diluted. 3. Please click on the links below to view the Microplate Reader Comparison Tables. The accurate positioning of 96-arrayed colonies on rectangular agar plates (in a 96-well scheme) was used to measure each colony with the optics of the plate reader. The reader is ideal for measuring flash and glow assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer (BRET), and acridinium ester flash assays, in both 96- and 384-well plates. Read one sample or up to 384 in a. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Fax 4200 BERTHOLD TECHNOLOGIES Lumat LB 9507 Tecan SpectraFluor Thermo Scientific Varioskan LUX LJL Biosystems Analyst AD 96. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. This can increase throughput and efficiency, thus saving time and space in the lab. CHO-M1 cells were plated at a density of 50,000 cells per well (96-well) or 12,500 cells per well (384-well) the day prior to assay. “The ABS Plus reader comes standard with our patented PathCheck® Technology which enables consistent results across a 96- or 384-well plate. 1536-well: 0. 03 pM 384-well: 0. This paper describes a robust and rapid procedure for measuring chlorophyll using a microplate reader. 5°C at 37°C. Figure 2. or ValitaTiter Plus (Molecular Devices cat. (96, 384, or 1536 wells) and the "Select Specific". The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. Page 154: Ordering Parts And Accessories. the SpectraMax i3x Multi-Mode Microplate Reader, may also be used for this application. This report demonstrates that the SPECTRAmax GEMINI-XS microplate spectrofluorometer, with its dual scanning monochromator and patented optical sensitivity, can be used to detect a relatively low number of cells expressing GFP. However, an upgradeable multi-mode reader which includes absorbance, fluorescence and luminescence can address all your future lab needs. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Standard SBS 96 and 384 well plates (128. Plate Formats: 6- to 384-well plates, NanoQuant Plate (Sold Separately) Detection Modes: Luminescence, Fluorescence, Absorbance. *Simple analysis and optimization. 0 GB Brand Hewlett-Packard Hard driveThe microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. ©2010 molecular Devices, inc. Outer wells are aligned for proper viewing with FLIPR. Important things to consider when purchasing a multimode microplate reader. . SpectraMax Mini Multi-Mode Microplate Reader Afordable multi-mode microplate reader with best-in-class data analysis software Perform your favorite applications including. This unit offers a wavelength range of 340 nm to 850 nm and a bandwidth of 5 nm. 1. 1 sec. F3/F5 Multi-Mode Microplate ReadersBulk Kit (10-plate size, Molecular Devices P/N R8347) HeLa cells (ATCC P/N CCL-2) Staurosporine (Sigma P/N S5921) 96-well black, clear-bottom microplates (Corning P/N 3904) SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. microplates or in a cuvette at a selected. 3% in white 96- and 384-well microplates Time-Resolved Fluorescence Performance (M4, M5, M5e only) Reading capabilities Top or bottom of a microplateWide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. Microplate readers from Molecular Devices are designed to provide consistent performance over a long period of time. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. However, an upgradeable multi-mode reader which includes absorbance, fluorescence and luminescence can address all. Highly selective for dsDNA over ssDNA or RNA. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. A preconfigured. #ID3-STD) • L-Tryptophan (Sigma cat. Products : SpectraMax M2/M2e reader, SpectraMax. Equipped with scanning, shaking and kinetics modules. We would like to show you a description here but the site won’t allow us. Insert the filled microplate into the drawer, matching well A1 with position A1. The reader can measure flat and round 96-well microplates and ensure accuracy by automatic lamp calibration before each reading. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Absorbance was read at 480 nm on the SpectraMax ABS Plus reader. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. Other Molecular Devices readers with absorbance detection mode, e. Both readers were heated to 37ºC prior to setting up . validation, maintenance, and repair documentation. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Optimized optical path - The reader’s unique quartz fiber optic system minimizes stray light for precise readings across a microplate. Unparalleled performance on a personalized platform. 3. Documentation:1. You can mix the contents of the wells in a microplate automatically by shaking before each read cycle. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. 96- or 384-well microplate compatibility. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. molecular devices gmbh tel. 96-well microplate; Immunoassay analyzer;VersaMax price. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. the assay. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. Flexibility to read a variety of plate formats. Visible absorbance -. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate. 2 W x 44. The microplate reader then generates data following a preconfigured protocol that performs the necessary calculations on the data, delivering consistent results. NanoRacks added it to their line of commercial . The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. Learn more 0200-7008 6- to 1536-well plates. The iMark. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. The VersaMax reads 96-well microplates. Microplates . section, select plate section titled. Note: SoftMax Pro Software must be installed on a computer connected to the instrument. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. 10. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. Step 1: Turn on your microplate reader. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 4 Pages. 1 OD at 230 nm. Reads standard cuvettes & 96-384 well plates from 190 to 1000 nm. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. Fluorescence Microplate Reader Comparison Chart. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. LUMINESCENCE MICROPLATE READING: Molecular Devices: SpectraMax L: Thermo Fisher Scientific: Luminoskan Ascent:. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. +49-89/96-05-88-0 Fax +49-89/9-62-02-34-5 Japan. The drawing shows a standard microplate of 15 mm height covered with a lid. Specifications for the SpectraMax L microplate reader. Three low volume 24-well bottom slides. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. SpectraMax ABS Plus reader utilizes Molecular Devices PathCheck Sensor- the only patented. A. Three kits covering a wide range of dsDNA concentrations. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903). EarlyTox Live/Dead Assay Kit (Molecular Devices cat. 0 OD Photometric resolution: 0. . #I2511) • Assay diluent – may be cell culture medium. #N207) LAL Reagent Water (Lonza cat. absorbance, fluorescence, and luminescence with the added functionality of. Software Validation. #T-0254) • Lysozyme (Sigma cat. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. If the best wavelengths are unknown, the Gemini EM reader will find. Application Area: Spectrophotometer for molecular biology. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. The absorbance of the tested solutions was measured at 405 nm using a SpectraMax® ABS PLUS Absorbance Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. Bioz Stars score: 86/100, based on 1 PubMed citations. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. Average StDev (mP) 1. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Published Dec 16, 2020 | Updated Dec 16, 2020. Bioz Stars score: 86/100, based on 1 PubMed citations. Contents of wells were pipetted up and down several times to mix. The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS Plus Microplate Reader, which can read a 96-well microplate in less than 10 seconds. Click on the crosshair and move it so that it is centered over the well image. The purposes of the study were: 1) to determine the optimal wavelength settings for three cell lines. A microplate reader as . 8. Vrla used the device to quantify the concentration of a small molecule produced by. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . 03 pM. Microplate reader assays requiring infrared (IR) or red-shifted. Infinite 200 Pro Microplate Reader. Also refer to this Multimode Reader Comparison Chart. For more sample throughput, the system can be. Make sure to Data 96孔微孔板的数据对比. adjust the reader. Molecular Devices' microplate readers. When reading at wavelengths. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. Wide range of applications The VersaMax reader covers. Description. I contacted Molecular Devices and they quoted me almost $6k to come out and replace. In combination with the SpectraMax Mini Multi-Mode Microplate Reader, it enables increased throughput and detection of DNA as low as 2 ng/μL using just a few microliters of sample. automation for any lab. Create custom fluorescence assays without having to purchase expensive filter sets. For more sample throughput, the system can well. 030 fmol Fluorescence Polarization (FP). Pick the best speed, sensitivity, and price for your needs. We would like to show you a description here but the site won’t allow us. We would like to show you a description here but the site won’t allow us. This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. rhodamine-like labels, using specific LED and EX/EM filters • 6- to 1536-well plates 0200. VANTAstar™ Flexible Multi-mode Microplate Reader. In . The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader.